Journal: Cell Reports Medicine

Article Title: Selective apoptosis of tumor-associated platelets boosts the anti-metastatic potency of PD-1 blockade therapy

doi: 10.1016/j.xcrm.2025.101984

Figure Lengend Snippet:

Article Snippet: Mouse SELP (P-Selectin) ELISA Kit , Elabscience , CatE-EL-M0638.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software, Staining, Modification, Clinical Proteomics, Membrane

Journal: Molecular medicine reports

Article Title: Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair.

doi: 10.3892/mmr.2016.5294

Figure Lengend Snippet: Figure 4. mRNA expression levels of TIMP‑2, MMP‑2 and VEGF in M2 macrophages. mRNA levels were determined by reverse transcription‑quan titative polymerase chain reaction analysis, and protein expression levels were determined by ELISA. Data are presented as the mean ± standard deviation. **P<0.05, compared with eIF6+/+ M2 macrophages. eIF6, eukaryotic initia tion factor 6; TIMP, tissue inhibitor of metalloproteinase‑2; MMP‑2, matrix metalloproteinase‑2; VEGF, vascular endothelial growth factor.

Article Snippet: The supernatant obtained from the culture medium of macrophages following stimulation with IL-4 for 24 h was collected, and the expression levels of VEGF (cat. no. EK0506), MMP-2 (cat. no. EK0511) and TIMP-2 (cat. no. EK0322) were detected using ELISA kits (Boster Biological Technology Co., Ltd., Wuhan, China), in strict accordance with the manufacturer's protocol.

Techniques: Expressing, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Journal of Translational Medicine

Article Title: Proteomic insights into platelet dysregulation and pathogenic mechanisms of chronic thromboembolic pulmonary hypertension

doi: 10.1186/s12967-025-06891-8

Figure Lengend Snippet: Level of platelet P-selectin and platelet morphology. A The plot depicts the gating strategy used to identify platelet populations. The defined region indicates the platelet population determined by size and complexity characteristics measured via flow cytometry. Platelet activation is evaluated by the percentage of CD41⁺ platelets that are also positive for P-selectin. B and C Expression levels of P-selectin in platelets ( B ) and its concentration in plasma ( D ) in healthy individuals and CTEPH patients. D Transmission electron microscopy (TEM) was employed to visualize the ultrastructure of platelets from a non-stimulated sample from CTEPH patients, specifically revealing the presence of dense granules. As shown in the TEM images, black arrows indicate the platelet-derived particles released by platelets, while red arrows point to the pseudopodia extended by activated platelets. Scale bars, 500 nm. Statistical significance was determined by Student's t-test. ** p value < 0.01; **** p value < 0.0001

Article Snippet: P-selectin was tested using PPP from healthy controls and CTEPH patients (Cat.CSB-E04708h, Cusabio, China).

Techniques: Flow Cytometry, Activation Assay, Expressing, Concentration Assay, Clinical Proteomics, Transmission Assay, Electron Microscopy, Derivative Assay

Journal: Journal of Translational Medicine

Article Title: Proteomic insights into platelet dysregulation and pathogenic mechanisms of chronic thromboembolic pulmonary hypertension

doi: 10.1186/s12967-025-06891-8

Figure Lengend Snippet: Platelet-mediated NET formation and its link to CTEPH thrombus resolution. A Flow cytometry demonstrates the identification of different populations of immune cells (granulocytes, monocytes, lymphocytes). Co-level of CD16 and CD66b is defined as neutrophils, and the proportion of CD41-positive groups in neutrophils is analyzed. B Quantification of CD41 + platelet-neutrophil aggregates in CTEPH patients compared to healthy controls. C Quantification of MPO-DNA complex protein in CTEPH patients compared to healthy controls. D Correlation between MPO-DNA complex levels and ITGB2 concentration in CTEPH patients. E Correlation between MPO-DNA complex levels and P-selectin concentration in CTEPH patients. F The left column displays Movat’ s staining under light microscopy, showing the early thrombus lysis stage, the thrombus fibrosis stage, and the neointima formation stage of CTEPH. The right column displays multiplex immunofluorescence, showing the immunostaining of CD41 (red), MPO (pink), citH3 (green), and nuclear staining (DAPI, blue). CD41, MPO, and citH3 were co-expressed in the early thrombus lysis stage, the thrombus fibrosis stage, and the neointima formation stage. Statistical significance was determined by Student's t-test. * p value < 0.05; ** p value < 0.01

Article Snippet: P-selectin was tested using PPP from healthy controls and CTEPH patients (Cat.CSB-E04708h, Cusabio, China).

Techniques: Flow Cytometry, Concentration Assay, Staining, Light Microscopy, Lysis, Multiplex Assay, Immunofluorescence, Immunostaining

Journal: Journal of Translational Medicine

Article Title: Proteomic insights into platelet dysregulation and pathogenic mechanisms of chronic thromboembolic pulmonary hypertension

doi: 10.1186/s12967-025-06891-8

Figure Lengend Snippet: The mechanism of platelet involvement in CTEPH. Platelets first adhere to the injured endothelial surface, become activated in response to signals such as P-selectin and other cytokines (e.g., HMGB1), leading to the release of inflammatory molecules. The increase of platelet NOX2 leads to the increase of ROS and promotes the formation of neutrophil extracellular trap (NET). Then the interaction between activated platelets and neutrophils through integrin β2(ITGB2) results in the formation of platelet-neutrophil aggregates. Platelet-neutrophil aggregates release DNA, PAD4, cathepsin G and intracellular substances in the form of NETs. Moreover, under the action of PAD4, the interaction between neutrophil-derived DNA and MPO forms the MPO-DNA complex, further promoting inflammation and coagulation. Activated platelets and the MPO-DNA complex contribute to endothelial dysfunction, which delayed thrombus resolution. Abbreviations : NOX2, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2; ROS, reactive oxygen species; PAD4, peptidylarginine deiminase 4; MPO, myeloperoxidase; HMGB1, high mobility group box-1 protein; EC, endothelial cell

Article Snippet: P-selectin was tested using PPP from healthy controls and CTEPH patients (Cat.CSB-E04708h, Cusabio, China).

Techniques: Derivative Assay, Coagulation